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home > people - current lab members > Pasquale Stano

Dr. Pasquale Stano

Dipartimento di Scienze
Università degli Studi di Roma Tre
Viale Marconi 446
00146 Roma

Tel.: +39.06.5733 6433 (Lab -2B); +39.06.5733 6363 (Lab 2.4)
Fax: +39.06.5733 6321
e-mail : pasquale.stano @ uniroma3.it

This webpage has been revised on: 2014-July-18

Current position: Scientific Guest at the Science Department of the Roma Tre University

Pasquale Stano received his scientific education in chemistry at the University of Pisa, Italy. In his thesis, done under the supervision of Prof. Mauro Isola, he worked on the design and synthesis of heterotopic Schiff base ligands and relative nickel and copper supramolecular complexes, and studied the kinetics of trans-imination on these substrates.

He joined Luisi's group in Zürich from February 2002, working on the liposomal drug delivery (camptothecin derivatives, Sigma-Tau, Pomezia, IT). Since 2003 he was working on the properties and reactivity of vesicles (effect of Trp-oligopeptides, turbidimetric analysis, cationic vesicle formation), DNA condensation in reverse micelles, matrix effect and self-reproduction of vesicles, and on the AmB interaction with sterol-containing vesicles.
In 2004 he moved to the Dept. of Biology of the University of Roma Tre (Rome, Italy), where is involved in the Luisi's project dealing with the physico-chemical properties of vesicles and other compartments, and their use as cell models.

In the last nine years he worked mainly in the "minimal cell" project, dealing with the construction of synthetic minimal cells for origin of life studies and biotechnology. He has been involved in several national and international projects (3 years Junion Grant at the "Enrico Fermi" Centre, for a project on synthetic cells; 2 years PRIN 2008FY7RJ4, 3.5 years FP6 "Synthcells" project nr. 043359, 3 years HFSP Nr. RGP0033/2007-C). This work is based on the development of methods based on liposome technology, cell-free protein synthesis. Recent research is focused on encapsulation of macromolecules inside vesicles and on the study of vesicle "diversity" due to stochastic phenomena. He is also collaborating with groups that perform mathematical models on vesicle and cell free systems (Fabio Mavelli, Univ. Bari; and Roberto Marangoni, Univ. Pisa). A new research direction has been recently started, aimed at constructing synthetic cells capable of communicating with biological cells (in collaboration with Giordano Rampioni, Univ. RomaTre). Moreover, he has been involved in additional projects on lecithin organogel and cationic liposomes for drug delivery (in collaboration with the pharmaceutical company Sigma-Tau), reactivity of lipid and fatty acid vesicles (interaction between proteins and vesicles, vesicle fusion, vesicle self-reproduction, phosphatidic acid vesicles), as well as in biochemical projects mainly on characterization, by spectroscopic methods (fluorescence and CD) of flavoenzymes (amino oxidase) and of random proteins (in collaboration with Cristiano Chiarabelli). Additional collaborations involve the use of flow-cytometry to characterize vesicles.

In 2007, he has co-edited a special issue of Origins of Life and Evolution of Biospheres on "Basic Questions about the Origins of Life (proceedings of the 4th Course of the International School on Complexity - Erice - 1-6 October 2006)", a special issue of Synthese on "Philosophical and scientific perspectives on emergence" (proceedings of the 9th Course of the International School on Complexity - Erice - 12-16 April 2009). Moreover, he co-edited a book on Minimal Cell (Springer). Currently he is involved in two more special issues: the first for Current Opinions in Chemical Biology, in a special issue on Synthetic Biology; the second for MDPI Life, in a special issue on Protocells. He is/has been member of the International Liposome Society, Italian Society of Pure and Applied Biophysics (SIBPA), American Chemical Society, Artificial Life Society.

Pasquale Stano is author and co-authors of several articles, reviews, book chapters, proceeding papers on origins of life, synthetic biology, biochemistry, physical chemistry. He acted as reviewer for about 180 manuscripts and ten research projects in his fields of expertise.

He has got the National Scientific Abilitation in Organic Chemistry (03/C1) and Biochemistry (05/E1) in December 2013 and June 2014, respectively ASN.

"A chemist following natural evolution" - interview with Pasquale on Science Careers.

Article Young Italian Scientists Take to the Streets, article on Science Careers featuring Pasquale's job perspectives

Courses at Uniroma3:
Applied Biochemistry and Molecular Biology (Module II: spectroscopy methods in biochemistry), from AA 2004-05 to AA 2010-11

Courses at Università of Venice
Ca' Foscari Physical Chemistry of Colloids (Module II: lipid vesicles: preparation, properties and applications); from AA 2008-09 to AA 2010-11.

Master in Nanotechnology 2008 (ICN Venice 2009)
Liposomes in Basic and applied Science


102. Walde, P.; Umakoshi, H.; Stano, P.; Mavelli, F.
Emergent properties arising from the assembly of amphiphiles. Artificial vesicle membranes as reaction promoters and regulators.
Chemical Communications, doi: 10.1039/c4cc02812k.

101. Le Chevelier Isaad, A.; Carrara, P.; Stano, P.; Krishnakumar, K. S.; Lafont, D.; Buchet, R.; Bouchu, D.; Albrieux, F.; Strazewski, P.
A hydrophobic disordered peptide spontaneously anchors a covalently bound RNA hairpin to giant lipidic vesicles.
Organic & Biomolecular Chemistry, doi: 10.1039/C4OB00721B.

100. Rampioni, G.; Mavelli, F.; Damiano, L., D’Angelo, F.; Messina, M.; Leoni, L.; Stano, P.
A synthetic biology approach to bio-chem-ICT: first moves towards chemical communication between synthetic and natural cells.
Natural Computing, doi: 10.1007/s11047-014-9425-x.

99. Stano, P.
Origin of Life Research.
In: Discoveries in Modern Science: Exploration, Invention, Technology, J. Trefil (Ed.), Macmillan Reference, in press.

98. Stano, P.; Souza, T.; Carrara, P.; Altamura, E.; D’Aguanno, E.; Caputo, M.; Luisi, P. L.; Mavelli, F.
Recent biophysical issues about the preparation of solute-filled lipid vesicles.
Mechanics of Advanced Materials and Structures, doi: 10.1080/15376494.2013.857743.

97. Cervelli, M.; Angelucci, E.; Stano, P.; Leboffe, L.; Federico, R.; Antonini, G.; Mariottini, P.; Polticelli, F.
The Glu216-Ser218 pocket is a major determinant of spermine oxidase substrate specificity.
Biochemical Journal, 2014, 461, 453-459.

96. Piloto Ferrer, J.; Cozzi, R.; Cornetta, T.; Stano, P.; Fiore, M.; Degrassi, F.; De Salvia, R.; Remigio, A.; Francisco, M.; Quiñones, O.; Valdivia, D.; González, M. L.; Sánchez-Lamar, A.; Pérez, C.; Sánchez-Lamar, A.
Xanthium strumarium L. extracts produce DNA damage mediated by cytotoxicity in in vitro assays but does not induce micronucleus in mice.
BioMed Research International 2014, 2014, 575197.

95. Chiarabelli, C.; Stano, P.; Luisi, P. L.
Chemical Synthetic Biology projects: Never Born Biopolymers and Synthetic Cells.
In: Synthetic Biology (Vol. 1), M. Ryadnov, L. Brunsveld, H. Suga (Eds.), Royal Society of Chemistry, 2014, 1, 292-329.

94. Mavelli, F.; Altamura, E.; Cassidei, L.; Stano, P.
Theoretical approaches to minimal artificial cells.
Entropy 2014, 16, 2488-2511.

93. Ahou, A.; Martignago, D.; Alabdallah, O.; Tavazza, R.; Stano, P.; Macone, A.; Pivato, M.; Masi, A.; Rambla, J. L.; Vera-Sirera, F.; Angelini, R.; Federico, R.; Tavladoraki, P.
A plant spermine oxidase/dehydrogenase regulated by proteasome and polyamines.
Journal of Experimental Botany 2014, 65, 1585-1603.

92. Adamala, K., Anella, F.; Wieczorek, R.; Stano, P.; Chiarabelli, C.; Luisi, P. L.
Open questions in origin of life: experimental studies on the origin of nucleic acids and proteins with specific and functional sequences by a chemical synthetic biology approach.
Computational and Structural Biotechnology Journal 2014, 9, e201402004.

91. Stano, P.; Rampioni, G.; Damiano, L.; D’Angelo, F.; Carrara, P.; Leoni, L.; Luisi, P. L.
Towards the engineering of chemical communication between semi-synthetic and natural cells.
In “Evolution, Complexity and Artificial Life” (ISBN 978-3-642-37576-7), S. Cagnoni, M. Mirolli, M. Villani (Eds.), Springer, Dordrecht, 2014, pp. 91-104.

90. Stano, P.; D’Aguanno, E.; Bolz, J.; Fahr, A.; Luisi, P. L.
A remarkable self-organization process as the origin of primitive functional cells.
Angewandte Chemie Int. Ed. Engl. 2013, 52, 13397-13400.

89. Chiarabelli, C.; Stano, P.; Luisi, P. L.
Chemical synthetic biology: a mini-review.
Frontiers in Microbiotechnology, Ecotoxicology and Bioremediation 2013, 4, 285.

88. Rampioni, G.; Damiano, L.; Messina, M.; D’Angelo, F.; Leoni, L.; Stano, P.
Chemical communication between synthetic and natural cells: a possible experimental design.
Electronic Proceedings in Theoretical Computer Sciences 2013, 130, 14-26.

87. Cabré, E. J.; Sánchez-Gorostiaga, A.; Carrara, P.; Ropero, N.; Casanova, M.; Palacios, P.; Stano, P.; Jiménez, M.; Rivas, G.; Vicente, M.
Bacterial division proteins induce vesicle collapse and cell membrane invagination.
J. Biol. Chem. 2013, 288, 26625-26634.

86. Grotzky, A.; Altamura, E.; Adamcik, J.; Carrara, P.; Stano, P.; Mavelli, F.; Nauser, T.; Mezzenga, R.; Schlüter, A. D.; Walde, P.
Structure and enzymatic properties of molecular dendronized polymer-enzyme conjugates and their entrapment inside giant vesicles.
Langmuir 2013, 29, 10831-10840.

85. Stano, P.; D’Aguanno, E.; Carrara, P.; Fahr, A.; Luisi, P. L.
Recent advancements in synthetic cells research.
In: Advances in Artificial Life, ECAL 2013: Proceedings of the Twelfth European Conference on the Synthesis and Simulation of Living Systems (2-6 September 2013, Taormina, Italy); P. Liò, O. Miglino, G. Nicosia, S. Nolfi, M. Pavone (Eds.), MIT Press (e-book, ISBN 978-0-262-31709-2) 2013, pp. 1160-1161.

84. Mavelli, F.; Caputo, M.; Altamura, E.; Stano, P.
Stochastic simulations of minimal cell model systems.
In: Proceedings of the 12th Joint European Thermodynamics Conference, JETC 2013, M. Pilotelli and G.P. Beretta (Eds), ISBN 978-88-89252-22-2, Snoopy, Brescia, Italy, 2013, pp. 73-81.

83. Scognamiglio, V.; Stano, P.; Polticelli, F.; Antonacci, A.; Dimova Lambreva, M.; Pochetti, G.; Giardi, M. T.; Rea, G.
Design and biophysical characterization of atrazine-sensing peptides mimicking the Chlamydomonas reinhardtii plastoquinone binding niche.
Phys. Chem. Chem. Phys. 2013, 15, 13108-13115.

82. Pesiri, V.; La Rosa, P.; Stano, P.; Acconcia, F.
Identification of an estrogen receptor alpha non-covalent ubiquitin binding surface: role in 17beta-estradiol-induced transcriptional activity.
Journal of Cell Science, 2013, 126, 2577-2582.

81. Stano, P.; Souza, T. P.; Kuruma, Y.; Carrara, P.; Luisi, P. L.
Semi-synthetic minimal cells: biochemical, physical and technological aspects.
In: Synthetic Biology: Tools and Applications. H. Zhao (Ed.), Academic Press-Elsevier, Amsterdam, 2013. Pp. 261-276.

80. Calviello, L.; Stano, P.; Mavelli, F.; Luisi, P. L.; Marangoni, R.
Quasi-cellular systems: stochastic simulation analysis at nanoscale range.
BMC Bioinformatics 2013, 14(Suppl. 7), S7.

79. Stano, P.; Luisi, P. L.
Construire une cellule de toutes pieces.
Les Dossiers de La Recherche 2013, 2, 54-57.

78. Stano, P.; Luisi, P. L.
Semi-synthetic minimal cells: Origin and recent developments.
Curr. Opin. Biotech. 2013, 24, 1-6.

77. Souza, T.; Stano, P.; Steiniger, F.; D'Aguanno, E.; Altamura, E.; Fahr, A.; Luisi, P. L.
Encapsulation of Ferritin, Ribosomes, and Ribo-Peptidic Complexes Inside Liposomes: Insights Into the Origin of Metabolism.
Orig. Life Evol. Biosph. 2012, 42, 421-428.

76. Di Donato, L.; Cataldo, M.; Stano, P.; Massa, R.; Ramundo-Orlando A.
Permeability changes of cationic liposomes loaded with carbonic anhydrase induced by millimeter waves radiation.
Radiation Research 2012, 178, 437-446.

75. Carrara, P.; Stano, P.; Luisi, P. L.
Giant vesicles 'colonies': a model for primitive cell communities.
ChemBioChem 2012, 13, 1497-1502.

74. Odorisio, T.; De Luca, N.; Vesci, L.; Luisi, P. L.; Stano, P.; Zambruno, G.; Pisano, C.
The atypical retinoid E-3-(3'-Adamantan-1-yl-4'-methoxybiphenyl-4-yl)-2-propenoic acid (ST1898) displays comedolytic activity in the rhino mouse model.
Eur. J. Dermatol. 2012, 22, 505-511.

73. Bersini, H.; Stano, P.; Luisi, P. L.; Bedau, M.
Philosophical and scientific perspectives on emergence.
Synthese 2012, 185, 165-169.

72. Bersini, H.; Stano, P.; Luisi, P. L.; Bedau, M. (Guest Editors)
Philosophical and scientific perspectives on emergence
(Special Issue: International School on Complexity - Emergence in the physical and biological world: a notion in search of clarification). Synthese 2012, Volume 185, Pp. 165-317.

71. Stano, P.; Rampioni, G.; Carrara, P.; Damiano, L.; Leoni, L.; Luisi, P. L.
Semi-synthetic minimal cells as a tool for biochemical ICT.
Biosystems 2012, 109, 24-34.

70. Chiarabelli, C.; Stano, P., Anella, F., Carrara, P., Luisi, P. L.
Approaches to chemical synthetic biology.
FEBS Letters 2012, 586, 2138-2145.

69. Stano, P.; Rampioni, G.; Damiano, F.; D'Angelo, F.; Carrara, P.; Leoni, L.; Luisi, P. L.
Experimental perspectives for a chemical communication between synthetic and natural cells.
Proceedings of the Italian Workshop on Artificial Life and Evolutionary Computation WIVACE (Parma, 20-21 February 2012); ISBN 978-88-903581-2-8 (Published on CD), S. Cagnoni, M. Mirolli, M. Villani (Eds.), 2012, Pp. 1-12.

68. Lazzerini-Ospri, L.; Stano, P.; Luisi, P. L.; Marangoni, R.
Characterization of the emergent properties of a synthetic quasi-cellular system.
BMC Bioinformatics, 2012 13, S9.

67. Stano, P.
Autopoietic self-reproduction as a distinctive feature of structural and dynamic organization in microcompartment systems: From self-assembled micelles to synthetic cells.
In: "Self-Healing at the Nanoscale: Mechanisms and Key Concepts of Natural and Artificial Systems", V. Amendola, M. Meneghetti (Eds.), CRC Press Taylor & Francis Group, Boca Raton, 2012. Pp. 333-356.

66. Stano, P.; Kuruma, Y.; Souza, T.; Carrara, P., Luisi, P. L.
Synthetic Biology and the Minimal Cell Project.
In: "4th European Conference on Chemistry for Life Sciences - Budapest, Hungary, August 31 - September 3, 2011", M. Kiss, A. Perczel (Eds.), Medimond, Pianoro (Italy), 2011. Pp. 109-112.

65. Stano, P.; Carrara, P.; Kuruma, Y.; Souza, T.; Luisi, P. L.
Compartmentalized reactions as a case of soft-matter biotechnology: Synthesis of proteins and nucleic acids inside lipid vesicles.
Journal of Material Chemistry, 2011, 21, 18887-18902.

64. Souza, T.; Steiniger, F.; Stano, P.; Fahr, A., Luisi, P. L.
Spontaneous crowding of ribosomes and proteins inside vesicles: A possible mechanism for the origin of cell metabolism.
ChemBioChem 2011, 12, 2325-2330.

63. Luisi, P. L.; Stano, P.
Minimal cell mimicry.
Nature Chem. 2011, 3, 755-756.

62. Stano, P.;
Advances in minimal cell models: A new approach to synthetic biology and origin of life.
In "Progress in Molecular and Environmental Bioengineering - From Analysis and Modeling to Technology Applications" A. Carpi (Ed.), Intech - Open Access Publisher, Rijeka, Croatia, 2011. Pp. 23-44.

61. Stano, P.; Carrara, P.; Souza, T., Luisi, P. L.
An update on the minimal cell project: From the physics of solute encapsulation to the experimental modeling of cell communities.
In: "Advances in Artificial Life, ECAL 2011: Proceedings of the Eleventh European Conference on the Synthesis and Simulation of Living Systems", T. Lenaerts, M. Giacobini, H. Bersini, P. Bourgine, M. Dorigo and R. Doursat (Eds.), MIT Press, Cambridge (MA), 2011. Pp. 779-780.

60. Stano, P.;
Minimal cells: Relevance and interplay of physical and biochemical factors.
Biotechnology Journal 2011, 6, 850-859.

59. Stano, P., Luisi, P. L
On the construction of minimal cell models in synthetic biology and origins of life studies.
In: "Design and Analysis of Biomolecular Circuits: Engineering Approaches to Systems and Synthetic Biology", H. Koeppl; G. Setti; M. Di Bernardo; D. Densmore (Eds.), Springer, Dordrecht, 2011; pp. 337-368.

58. Stano, P.; Ferri, F.; Luisi, P. L.
Semi-synthetic minimal living cells.
In: "Chemical Synthetic Biology", P. L. Luisi, C. Chiarabelli (Eds.) Wiley, Chichester, 2011. Pp. 247-286.

57. Norris, V.; Zemirline, A.; Amar, P.; Audinot, J. N.; Ballet, P.; Ben-Jacob, E.; Bernot, G.; Beslon, G.; Cabin, a.; Fanchon, E.; Giavitto, J.-L.; Glade, N.; Greussay, P.; Grondin, Y.; Foster, J. A.; Hutzler, G.; Jost, J.; Kepes, F.; Michel, O.; Molina, F.; Signorini, J.; Stano, P.; Thierry, A. R.
Computing with bacterial constituents, cells and populations: From bioputing to bactoputing.
Theory in Biosciences 2011, 130, 211-228.

56. Stano, P.; Souza, T. P.; Allegretti, M.; Kuruma, Y.; Luisi, P. L.
New and unexpected insights on the formation of protocells from a synthetic biology approach: The case of entrapment of biomacromolecules and protein synthesis inside vesicles.
In: "Minimal Cells", P. Stano, P. L. Luisi (Eds.) 2011, Springer, Dordrecht. Pp. 195-216.

55. Luisi, P. L. and Stano, P. (Eds.)
The Minimal Cell.
Springer, 2011. Springer, Dordrecht.

54. Tavladoraki, P.; Cervelli, M.; Antonangeli, F.; Minervini, G.; Stano, P.; Federico, R.; Mariottini, P.; Polticelli, F.
Probing mammalian spermine oxidase enzyme-substrate complex through molecular modeling, site-directed mutagenesis and biochemical characterization.
Amino Acids 2011, 40, 1115-1126.

53. Fabrini, F.; Bocedi, A.; Pallottini, V.; Canuti, L.; De Canio, M.; Urbani, A.; Marzano, V.; Cornetta, T.; Stano, P.; Giovanetti, A.; Stella, L.; Canini, A.; Federici, G.; Ricci, G.
Nuclear shield: A multi-enzyme task-force for nucleus protection.
Plos ONE 2010, 5, e14125.

52. Stano, P. ; Luisi, P. L.
Costruire une cellule de toutes pièces.
La Recherche 2010, 445, 48-51.

51. Luisi, P. L.; Allegretti, M.; Souza, T.; Steineger, F.; Fahr, A.; Stano, P.
Spontaneous protein crowding in liposomes: A new vista for the origin of cellular metabolism.
ChemBiochem 2010, 11, 1989-1992.

50. Stano, P.; Luisi, P. L.
Chemical approaches to synthetic biology: From vesicles self-reproduction to semi-synthetic minimal cells.
Artificial Life XII. Proceedings of the Twelfth International Conference on the Synthesis and Simulation of Living Systems. MIT press, Cambridge (MA), 2010. Pp. 147-153.

49. Stano, P.; Luisi, P. L.
Reactions in Liposomes.
In "Molecular Encapsulation: Organic Reactions in Constrained Systems" U. H. Brinker and J.-L. Mieusset (eds.) Wiley, 2010. Pp. 455-492.

48. Luisi, P. L., Ferri, F.; Stano, P.
Approaches to semi-synthetic minimal cells: a review. (Reprinted).
In: The Nature of Life: Classical and Contemporary Perspectives, M. Bedau and C. Cleland (eds.). Cambridge University Press, Cambridge, 2010. Pp. 272-288.

47. Stano, P.
Minimal and primitive cells in origins of life, systems chemistry and synthetic biology.
Orig. Life Evol. Biosphere 2010, 40, 452-457.

46. Bersini, H.; Stano, P.; Luisi, P. L.; Bedau, M. (eds.)
Philosophical and scientific perspectives on emergence.
Synthese 2010, DOI: 10.1007/s11229-010-9718-2.

45. Stano, P.
Synthetic biology of minimal living cells: protocells models and semi-synthetic cells.
Systems and synthetic biology 2010, 4, 149-156.

44. Stano, P.; Luisi, P. L.
Achievements and open questions in the self-reproduction of vesicles and synthetic minimal cells.
ChemComm 2010, 46, 3639-3653.

43. Caschera, F.; Stano, P.; Luisi, P. L.
Reactivity and fusion between cationic vesicles and fatty acid anionic vesicles.
Journal Colloid Interface Science 2010, 345, 561-565.

42. Walde, P.; Cosentino, K.; Hengel, H.; Stano, P.
Giant Vesicles: Preparations and Applications.
ChemBioChem 2010, 11, 848-865.

41. Mavelli, F.; Stano, P.
Kinetic models for autopoietic chemical systems: role of fluctuations in homeostatic regime,
Physical Biology 2010, 7, 016010.

40. Stano, P.; Kuruma, Y.; Souza, T. P., Luisi, P. L.
Biosynthesis of proteins inside liposomes.
Methods in Molecular Biology 2010, 606, 127-145.

39. Stano, P.
Cellule artificiali: dall'attuale quadro teorico-sperimentale al loro uso come robot molecolari.
In: Modelli, sistemi e applicazioni di Vita Artificiale e Computazione Evolutiva – Atti del Convegno WIVACE 2009. O. Miglino, M. Ponticorvo, A. Rega, F. Rubinacci (eds.); Fridericiana Editrice Universitaria, Napoli, 2009. Pp. 193-198.

38. Stano, P.; Luisi, P. L.
Semi-synthetic minimal cells.
In: Artificial Life and Evolutionary Computation: Proceedings of Wivace 2008; R. Serra, M. Villani, I. Poli (eds); World Scientific Publishing Co Pte Ltd., 2009, #24.

37. Stano, P.
Prove di vita minima.
Sapere 2009, 5, 20-27.

36. Stano, P.; Ruiz-Mirazo, K.
I quesiti di San Sebastián.
Sapere 2009, 5, 14-16.

35. Chiarabelli, C.; Stano, P.; Luisi, P. L.
Chemical Approaches to Synthetic Biology.
Curr. Opin. Biotech. 2009, 20, 492-497.

34. Stano, P.;
Systems Biology and Synthetic Biology. Edited by Pengcheng Fu and Sven Panke.
ChemBioChem 2009, 10, 2672-2673.

33. Zumbuehl, A.; Stano, P.; Sohrmann, M.; Dietiker, R.; Peter, M.; Carreira, E. M.
Synthesis and Investigation of Tryptophan–Amphotericin B Conjugates.
ChemBioChem. 2009, 10, 1617-1620.

32. Stano, P., Luisi, P. L.
Precellular Evolution: Vesicles and Protocells.
In: Prebiotic Evolution and Astrobiology. A. Lazcano and J. T. Wong (eds.), 2009, Landes Bioscience, Austin, Texas US; pp. 94-105.

31. Souza, T.; Stano, P.; Luisi, P. L.
The minimal size of liposome-based model cells brings about a remarkably enhanced entrapment and protein synthesis.
ChemBioChem, 2009, 10, 1056-1063.

30. Stano, P.; Murtas, G.; Luisi, P. L.
Semi-Synthetic Minimal Cells: New Advancements and Perspectives.
In: "Protocells. Bridging Nonliving and Living Matter". S. Ransmussen, M. A. Bedau, L. Chen, D. Deamer, D. C. Krakauer, N. H. Packard and P. F. Stadler (eds.); MIT Press, 2009, Cambridge, Massachusetts; pp. 39-70.

29. Stano P.,
Self-reproduction of vesicles and other compartments: a review.
In CRC Handbook of Surface and Colloid Chemistry – Third Edition – 2009, K. S. Birdi (ed.), CRC Press, Taylor and Francis Group, LLC, Boca Raton, USA; pp. 681-702.

28. Kuruma, Y.; Stano, P.; Ueda, T.; Luisi, P. L.,
A synthetic biology approach to the construction of membrane proteins in semi-synthetic minimal cells.
Biochim. Biophys. Acta 2009, 1788, 567-574.

27. Gorlero, M.; Wieczorek, R.; Adamala, K.; Giorgi, A.; Schininà, M. E.; Stano, P.; Luisi, P. L.
Ser-His catalyses the formation of peptides and PNAs.
FEBS Letters 2008, 583, 153-156.

26. Luisi, P. L.; Souza, T.; Stano, P
Vesicle behaviour: In search of explanations.
J. Phys. Chem. B 2008, 112, 14655-14664.

25. Stano, P.;
Approaches to the Construction of the Minimal Cell.
In: Proceedings of the Third International Conference "Smart Material Structures Systems" (CIMTEC 2008) Acireale 8-13/06/2008; P. Vincenzini and S. Graziani (Eds.). Advances in Science and Technology 2008, 58, 10-19.

24. Stano, P.; Luisi, P. L.
Self-reproduction of micelles, reverse micelles and vesicles. Compartments disclose a general transformation pattern.
In: Advances on Planar Lipid Bilayers and Liposomes, A. Leitmannova Liu (Ed.). 2008, Elsevier, Amsterdam, pp. 221-263.

23. Norris, V.; Zemirline, A.; Amar, P.; Ballet, P.; Ben-Jacob, E.; Bernot G.; Beslon, G.; Danos, V.; Giavitto, J.-L.; Greussay, P.; Hutzler, G.; Kepes, F.; Michel, O.; Misevic, G.; Molina, F.; Signorini, J.; Stano, P.; Thierry, A.
Bactoputing Perspective: Computing with bacteria.
In: "Proceedings of the Lille Spring School on Modelling Complex Biological Systems in the Context of Genomics. Lille, France". Poudret M.; Comet J.-P.; Le Gall P.; Képès F.; Arnould A.; Meseure P.; Verbavatz J.-M.; Rambourg A. (eds.). EDP Sciences, 2008, Les Ulis (Paris), pp. 133-160.

22. Spedaletti V.; Polticelli, F.; Capodaglio, V.; Schininà, M. E.; Stano, P.; Federico, R.; Tavladoraki, P.
Characterization of a lysine-specific histone demethylase from Arabidopsis thaliana.
Biochemistry 2008, 47, 4936-4947.

21. Marcocci, L.; Casadei, M.; Faso, C.; Antoccia, A.; Stano, P.; Leone, S.; Mondovì, B.; Federico, R.; Tavladoraki, P
Inducible expression of maize polyamine oxidase in the nucleus of MCF7 human breast cancer cells confers sensitivity to etoposide.
Amino Acids 2008, 34, 403-412.

20. Ramundo-Orlando, A; Gallerano, G. P.; Stano, P.; Doria, A.; Giovenale, E.; Messina, G.; Cappelli, M.; D'Arienzo, M.; Spassovsky, I.
Permeability changes induced by 130 GHz pulsed radiation on cationic liposomes loaded with carbonic anhydrase.
Bioelectromagnetics, 2007, 28, 587-598.

19. Fiordemondo, D.; Stano, P.
Lecithin-based water-in-oil compartments as dividing bioreactors.
ChemBioChem, 2007, 8, 1965-1973.

18. Luisi, P. L.; Stano, P.; Murtas, G.; Kuruma, Y.; Ueda, T.
En route to semi-synthetic minimal cells.
In: "Proceedings of the Bordeaux Spring School on Modelling Complex Biological Systems in the Context of Genomics. April 3rd - 7th 2006". P. Amar; F. Képès; V. Norris; M. Beurton-Aimar; J.-P. Mazat. EDP Sciences, 2007, Les Ulis (Paris), pp. 19-30.

17. Stano, P.
Question 7: New aspects of interactions among vesicles.
Origins of Life and Evolution of Biospheres. 2007, 37, 439-444.

16. Stano, P., Luisi, P. L.
Basic Questions About the Origins of Life: Proceedings of the Erice International School of Complexity (Fourth Course).
Origins of Life and Evolution of Biospheres. 2007, 37, 303-307.

15. Zumbuehl, A.; Stano, P.; Sohrmann, M.; Peter, M.; Walde, P.; Carreira, E. M.,
A novel strategy for bioconjugation: Synthesis and preliminary evaluation with amphotericin B.
Organic & Biomolecular Chemistry 2007, 5, 1339-1342.

14. Luisi, P. L.; Chiarabelli, C.; Stano, P.
From the Never Born Proteins to the Minimal Living Cell: Two Projects in Synthetic Biology.
Origins of Life and Evolution of the Biosphere 2006, 36, 605-616.

13. Chiarabelli, C. ; Vrijbloed, J. W.; De Lucrezia, D. ; Thomas, R. M. ; Stano, P. ; Polticelli, F. ; Ottone, T.; Papa, E., Luisi, P. L.
Investigation of de novo totally random biosequences. Part II. On the folding frequency in a totally random library of de novo proteins obtained by phage display.
Chemistry & Biodiversity 2006, 3, 840-859.

12. Sanna, C.; La Mesa, C.; Mannina, L.; Stano, P.; Viel, S.; Segre, A. L.
A new class of aggregates in aqueous solution: NMR, thermodynamic and light scattering study.
Langmuir 2006, 22, 6031-6041.

11. Stano, P.; Ferri, F.; Luisi, P. L.
From the minimal genome to the minimal cell: theoretical and experimental investigations.
In "Life as we know it (Series: Cellular Origin, Life in Extreme Habitats and Astrobiology; vol. 10)", ed. J. Seckbach, Springer, Berlin, 2006, pp. 181-198.

10. Stano, P.; Wehrli, E.; Luisi, P. L.
Insights on the self-reproduction of oleate vesicles.
Journal of Physics: Condensed Matter 2006, 18, S2231-S2238.

9. Ruiz-Mirazo, K.; Stano, P.; Luisi, P. L.
Lysozyme effect on oleic acid/oleate vesicles.
Journal of Liposome Research 2006, 16, 143-154.

8. Luisi, P. L.; Ferri, F.; Stano, P.
Approaches to a semi-synthetic minimal cell: a review.
Naturwissenschaften 2006, 93, 1-13.

7. Osfouri, S.; Stano, P.; Luisi, P. L.
Condensed DNA in lipid microcompartments.
Journal of Physical Chemistry B 2005, 109, 19929-19935.

6. Stano, P.; Bufali, S.; Domazou, A.; Luisi, P. L.
Effect of Trp oligopeptides on the size distribution of POPC liposomes. A dynamic light scattering and turbidimetric Study.
Journal of Liposome